Artificial skin and a preparation method thereof

A preparation method for artificial skin, comprising the following steps:

• S1. preparing a xenogeneic acellular dermal matrix particles solution, transferring the solution into a printing syringe, vacuuming to remove bubbles, and assembling to obtain a xenogeneic acellular dermal matrix gel, wherein the xenogeneic acellular dermal matrix gel comprises the xenogeneic acellular dermal matrix particles, hyaluronic acid, and a solvent;
• the particle size of the xenogeneic acellular dermal matrix particles is 10-400 μm, and the concentration of the xenogeneic acellular dermal matrix particles is 10-30% as a mass percentage of the xenogeneic acellular dermal matrix gel;
• the concentration of the hyaluronic acid is 0.1-2% as a mass percentage of the xenogeneic acellular dermal matrix gel;
• the assembling process is performed at a temperature of 0-37° C. and a humidity of 40%-80% for 12-48 h;
• S2. constructing three-dimensional model of dermis layer, setting printing parameters, and printing xenogeneic acellular dermal matrix gel obtained in S1, and then treating the printed products with processes of alcohol soaking, freeze-drying, glutaraldehyde vapor crosslinking, thermal crosslinking, and glutaraldehyde removal to obtain the dermis layer, wherein the alcohol soaking process is performed in 10-20% (v/v) alcohol at 0-4° C. for 12-48 h;
• the glutaraldehyde vapor crosslinking process is performed in 10-30% glutaraldehyde vapor at 37-52° C. for 2-5 h, the thermal crosslinking process is performed in 10-150 Pa condition at 100-110 ° C. for 12-48 h;
• the glutaraldehyde removing process is performed at 37-52° C. for 2-5 d;
• and
• S3. combining dermis layer obtained in S2 with an epidermis layer to obtain the artificial skin.