Ischaemia-free organ perfusion device and perfusion method

An ischaemia-free organ perfusion device, comprising:

• a first container for storing an isolated organ, wherein the first container is provided with a first perfusion port, a second perfusion port and a third perfusion port;
• a second container for recovering blood of an organ in donor acquisition stage and storing perfusate, wherein the second container is provided with a first port and a second port;
• a first flow path, comprising
• a first branch, wherein the first branch is configured for communicating the first perfusion port with the first port, and is provided with a first pump configured for flowing the perfusate from the first port to the first perfusion port, and
• a second branch, wherein the second branch is configured for communicating the second perfusion port with the first port, and is provided with a second pump configured for flowing the perfusate from the first port to the second perfusion port,
• a second flow path for communicating the third perfusion port with the second port;
• a third flow path for communicating the organ in donor acquisition stage with the first port;
• a fourth flow path for communicating the organ in donor acquisition stage with the second port;
• a first puncture catheter, wherein one end of the first puncture catheter is configured for connecting with the second perfusion port, and the other end of the first puncture catheter is configured for inserting into an artery of the organ in donor acquisition stage, so that the perfusate is perfused into the artery;
• a second puncture catheter, wherein one end of the second puncture catheter is configured for connecting with the first perfusion port, and the other end of the second puncture catheter is configured for inserting into a second vein of the organ in donor acquisition stage, so that the perfusate is perfused into the second vein;
• a venipuncture catheter, comprising
• a venous catheter lumen, wherein in donor acquisition stage, one end of the venous catheter lumen is configured for communicating with the fourth flow path, and the other end of the venous catheter lumen is configured for inserting into a first vein of the organ, so that the blood of the organ is recovered;
• and
• an auxiliary catheter lumen, wherein in donor acquisition stage, one end of the auxiliary catheter lumen is configured for communicating with the third flow path, and the other end of the auxiliary catheter lumen is configured for inserting into the first vein of the organ, so that the perfusate is perfused into the first vein;
• wherein, in isolation stage, the venipuncture catheter is capable of being cut off and a part of the venipuncture catheter inserted into the first vein is capable of communicating with the third perfusion port, so that the perfusate in the organ is recovered;
• wherein, the second container, the third flow path, the fourth flow path and the venipuncture catheter are capable of forming a second circulation with the organ in donor acquisition stage to perfuse the organ in donor acquisition stage and recover the blood of the organ;
• and after the organ is isolated, the second circulation is capable of being switched to a first circulation formed by the second container, the first flow path, the second flow path, the first puncture catheter, the second puncture catheter, the venipuncture catheter and the isolated organ in the first container to perfuse the isolated organ in preservation stage, and
• a third pump for providing power to the second circulation;
• wherein, uninterrupted blood supply for the transplanted organ during whole organ transplantation is maintained by normothermic machine perfusion.