Method and apparatus for keeping artificially created tissues alive

An apparatus comprising a culture chamber keeping artificially created tissues alive;

• a receptacle that is telescopically increased in its height, robotically, to match the three-dimensional bio-fabrication process of tissue structures or bio-stimulatory scaffold;
• and the ex vivo robotically controlled systems that aid by stimulating and supporting of the regenerative processes;
• the diameter of distance across the top of each section, ideally, is 2 mm larger than the section following below its position;
• the diameter of distance across the bottom of each section, ideally, is 4 mm smaller than its top opening;
• except for the top and bottom sections, the wall of each section, ideally, is 9 mm in height and tapered to meet the 4 mm difference in diameter between its upper and lower openings;
• the very top section, ideally, is 3 mm taller than the middle sections to provide for protruding lipped structure used to catch hold of clamps that hold it in place for its vertical lifting;
• two clamps for lifting are constructed with inner facing surfaces shaped to the contour of the wall of the culture chamber's base the top section lip also provides for the snug fit of a cap for culture chamber, which can be applied when the initial fabrications stages are complete at the tissue's maturation stage, the cap affixed to the top section has an entry port in its top for a tube that connects to the bio-fabricating tissue's main input/output vessel, the tube may have adjustable sliding clamps/locking spacers on both sides of the cap that allow modifiable depths of the tube's descent into the culture chamber;
• the very top section also has an input port with an outflow tube that enters at an angle parallel to the culture chamber's base, the tubes' openings are manufactured to fit flush with this section's inner wall, allowing its smooth telescopic movement across the outer wall of the lower connecting section, this port allows for bottom up extraction/out flow of contents surrounding the bio-fabricating tissue structures;
• the wall of the bottom section is cupped with an inner depth of 7 mm, ideally, and an outer height of 9 mm, ideally;
• the bottom section is attached to an appropriate base at the time of the culture chamber's assembly;
• the attachment of the base, done after all of the tapered walled sections are placed in the order of their width, within each other, hold all the sections in place;
• the base must at least be 4 mm wider that the lower opening of the top section;
• the bottom of the base is shaped to match the specimen mounting mechanism centrally located on the robotic production stage;
• the base may have a wall with an inner depth of 7 mm;
• the base may have two inlet tubes that enter the culture chamber perpendicular from the bottom and one outlet tube that enters at a right angle from the side. The side outlet tube transverses the wall of culture chamber's base and enters the wall of the culture chamber within a few millimeters of its bottom;
• the side tube protrudes slightly into culture chamber's open area, allowing a regulated, top-down extraction/out flow of contents surrounding the bio-fabricating tissue structures;
• made from cost effective materials anything from biodegradable glucose polymers, protein polymers, glass or plastics, to stainless steel, may be transparent, to allow during the tissue structures maturation phase, the laser manipulation of chemicals, materials and cells, robotically placed within the culture chamber during the structure's initial fabrication processes, or being supplied to the bio-fabrication site during tissues maturation phase through entry ports;
• or opaque to prefer light's effect on the bio-fabrication of the maturing tissue structures;
• culture chamber upright and in its expanded state the interior interfaces the inflow and outflow tubes are constructed and function within the culture chamber, done while simultaneously providing for the exchange of liquid mediums and materials within the fabricated structure and in the areas surrounding the tissue structure's production;
• culture chamber does this while providing for the tissue's support and maturation during the bio-fabrication process, also assisting in regulation of the buoyance of structural materials added to the culture chamber by allowing changing of fluids of variable viscosities, supplied to the production area by peripheral devices, computer regulated and prearranged by the tissue's Bio-blueprint.