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US 11,519,917 B2Granted
Assay for quantifying polyphosphates
AssigneesThe Regents of the University of Michigan, The Board of Trustees of the University of Illinois
InventorsJames H. Morrissey, Stephanie A. Smith, Catherine J. Baker, Rachel Hemp, Richard J. Travers
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Abstract
Provided herein are compositions and methods for quantifying polyphosphates. In particular, provided herein are solution and substrate based assays for quantifying polyphosphates in complex samples.
Claims19
A method of detecting polyphosphate in a sample, comprising:
- a) contacting a sample comprising polyphosphate with an agent that inhibits binding of said polyphosphate to proteins in said sample;
- b) contacting said sample with a solid support coated in a polymer under conditions such that said polyphosphate binds to said polymer;
- c) washing said solid support to remove proteins in said sample;
- d) contacting said solid support with a polyphosphate binding protein under conditions such that said polyphosphate binding protein binds to polyphosphate;
- and
- e) quantifying the level of polyphosphate binding protein bound to said polyphosphate.
3 more elements
A method of detecting polyphosphate in a sample, comprising:
- a) contacting a sample comprising polyphosphate with an agent that inhibits binding of said polyphosphate to proteins in said sample and a known amount of labeled polyphosphate;
- b) contacting said sample with a solid support coated in a polymer under conditions such that said polyphosphate binds to said polymer;
- c) washing said solid support to remove proteins in said sample;
- and
- d) quantifying the level of labeled polyphosphate.
2 more elements
Dependencies are inferred from the claim text.