Metabolic disorder target
AssigneeUniversity of Pittsburgh—Of the Commonwealth System of Higher Education
InventorsNathan A. Yates, Steven James Mullett, Harris B. Bell-Temin
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Abstract
Methods are disclosed herein for identifying a compound of use in treating a condition treatable by metformin. The methods include determining if the test compound binds a subunit of the mitochondrial electron transport complex IV, and/or alters the function of the mitochondrial electron transport complex IV. Methods for treating a subject with a condition treatable by metformin, are also disclosed. In some embodiments, the condition is type II diabetes.
Claims11
A method for identifying a compound of use in treating a condition treatable by metformin, comprising:
- contacting a sample comprising a mitochondrial electron transport complex IV with a test compound;
- and
- determining if the test compound binds a subunit of the mitochondrial electron transport complex IV, wherein the subunit is Cox4i1, Cox5a, Cox6b1, Cox7a2, mt-Co2, Cox6c, Cox7c, Cox5b, or Cox6a1, and
- wherein binding of the test compound to the subunit or change in function of the mitochondrial electron transport complex IV, indicates that the compound is of use in treating the condition.
A method for identifying a compound of use in treating a condition treatable by metformin, comprising:
- contacting a sample comprising a mitochondrial electron transport complex IV with a test compound;
- and
- determining if the test compound binds a subunit of mitochondrial electron transport complex IV;
- wherein determining if the test compound binds the subunit of the mitochondrial electron transport complex IV comprises performing mass spectrometry, and
- wherein binding of the test compound to the mitochondrial electron transport complex IV indicates that the compound is of use in treating the condition.
Dependencies are inferred from the claim text.