Lateral flow assay device

A diagnostic kit for detecting the presence or quantity of one or more test analytes within a test sample taken from a skin surface of a mammal, the diagnostic kit comprising:

• a) a separate insert for a lateral flow device comprising a membrane fixed to a rigid support frame, the membrane having a thickness, a width, a length, and a bottom for contacting the skin surface, to obtain the test sample,
• b) a lateral flow assay device comprising a housing and an opening for accepting the separate insert to an interior of the housing, wherein the lateral flow assay device comprises an elution zone and a detection area, and wherein the separate insert is the elution zone, and not the detection area,
• c) a securing member comprising at least one adhesive surface portion on a bottom side of the securing member for releasably attaching the separate insert to the skin surface of the mammal, wherein the securing member comprises an expandable layer extending from the at least one adhesive surface portion on the bottom side of the securing member to apply pressure to a top side of the rigid support frame of the separate insert and thereby pressing the separate insert against the skin surface of the mammal, wherein the expandable layer is not in fluid communication with the separate insert.