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US 11,519,851 B2Granted
Monitoring of compounds
AssigneeC Technologies AB
InventorsCraig Harrison, Ramsey Shanbaky
Life of this patent
Estimating the term…
Abstract
The present invention relates to methods for determining the change in concentration of a substance in solution over time by continuously monitoring in real time. In particular, the present invention relates to methods for continuously monitoring the concentration of compounds during the manufacturing process of biomolecules.
Claims23
A method of determining the change in concentration of a substance in solution over time comprising:
- continuously monitoring in real time the ultraviolet spectrum of the substance by:
- measuring the spectrum in a flow through mechanism comprising a light source, a pathlength, a fibrette and a detector, wherein the pathlength is dynamically altered by moving the fibrette to a plurality of dynamically variable pathlengths such that multiple measurements of the spectrum are made at the dynamically variable pathlengths between the fibrette and the detector;
- wherein the pathlength is dynamically altered in response to real time measurements so that samples are measured without dilution or concentration of the sample;
- determining a concentration of the substance based on said measured spectrum;
- comparing the concentration of the substance over time;
- and
- generating a control signal in response to the comparison, wherein the control signal adjusts a biotechnology process based on changing process conditions indicated by the comparison.
4 more elements
A method of determining the change in concentration of a substance in solution over time comprising:
- monitoring in real time the ultraviolet spectrum of the substance at discrete time points by:
- measuring the spectrum in a flow through mechanism comprising a light source, a pathlength, a fibrette and a detector, wherein the pathlength is dynamically altered by moving the fibrette to a plurality of dynamically variable pathlengths such that multiple measurements of the spectrum are made at the dynamically variable pathlengths between the fibrette and the detector at the discrete time points;
- wherein the pathlength is dynamically altered in response to real time measurements so that samples are measured without dilution or concentration of the sample;
- determining a concentration of the substance based on said measured spectrum;
- comparing the concentration of the substance at the discrete time points;
- and
- generating a control signal in response to the comparison, wherein the control signal adjusts a biotechnology process based on changing process conditions indicated by the comparison.
4 more elements
A method of determining the lifetime of a membrane used in ultrafiltration comprising:
- continuously monitoring in real time the concentration of a substance in a solution that has passed through the ultrafiltration membrane by:
- measuring the ultraviolet spectrum of the substance in a flow through mechanism comprising a light source, a pathlength, a fibrette and a detector, wherein the pathlength is dynamically altered by moving the fibrette to a plurality of dynamically variable pathlengths such that multiple measurements of the spectrum are made at the dynamically variable pathlengths between the fibrette and the detector;
- wherein the pathlength is dynamically altered in response to real time measurements so that samples are measured without dilution or concentration of the sample;
- determining a concentration of the substance based on said measured spectrum;
- comparing the concentration of the substance over time;
- and
- generating a control signal in response to the comparison, wherein the control signal adjusts a biotechnology process based on changing process conditions indicated by the comparison.
4 more elements
Dependencies are inferred from the claim text.