Paper-based colorimetric sensor kit for quickly and simply diagnosing mercury in situ with naked eye and method for quickly and simply detecting mercury in situ with naked eye using the same
AssigneeKorea Food Research Institute
InventorsMin-Ah Woo, Min-Cheol Lim, Sung-Wook Choi, Tai-Yong Kim
Life of this patent
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Abstract
There is provided a paper-based colorimetric sensor kit for quickly and simply diagnosing mercury in situ with a naked eye. The paper-based colorimetric sensor kit includes: a circular template for rolling circle amplification (RCA); a primer that does not hybridize to the circular template when a mercury ion is bonded to a primer that hybridizes to the circular template; a DNA polymerase; a sensing material kit including a nanoparticle probe labeled to a DNA coil formed in the circular template for RCA; and a radial chromatography paper.
Claims9
A paper-based colorimetric sensor kit for quickly and simply detecting a mercury ion in situ with a naked eye, the paper-based colorimetric sensor kit comprising:
- a circular template for rolling circle amplification (RCA);
- a primer that hybridizes to the circular template and does not hybridize to the circular template when a mercury ion is bonded to the primer;
- a DNA polymerase;
- a sensing material kit including a nanoparticle probe attached to a single-stranded oligonucleotide that is capable of hybridizing to a single-stranded DNA coil formed from the circular template when the circular template is amplified by RCA;
- and
- radial chromatography paper.
A method for quickly and simply detecting a mercury ion in situ with a naked eye, the method comprising:
- mixing and reacting a detection solution with a primer that hybridizes to a circular template for rolling circle amplification (RCA) and does not hybridize to the circular template when a mercury ion is bonded to the primer;
- mixing the circular template for RCA, a DNA polymerase, a nanoparticle probe attached to a single-stranded oligonucleotide that is capable of hybridizing to a single-stranded DNA coil formed from the circular template when the circular template is amplified by RCA, and dNTP in the mixed solution to perform an RCA reaction;
- adding the solution in which the RCA reaction is completed dropwise to a radial chromatography paper and then drying the solution;
- and
- observing concentric circles formed on the radial chromatography paper.
Dependencies are inferred from the claim text.