Artificial metalloenzymes containing noble metal-porphyrins

A catalyst composition comprising:

• a porphyrin;
• M(L), wherein the porphyrin and M(L) form a complex;
• M is a metal, wherein the metal is Ir;
• L is a ligand selected from the group consisting of alkyl, haloalkyl, halogen, CO, and CN;
• and
• a heme apoprotein, wherein the porphyrin-M(L) complex is bound to the heme apoprotein, wherein the heme apoprotein comprises at least one mutation, wherein the at least one mutation is close to an active site of the heme apoprotein,
• wherein the heme apoprotein is a cytochrome P450 or a myoglobin, and
• wherein the cytochrome P450 has at least 70% sequence identity to the P450 region of SEQ ID NO:3 and comprises a substitution of C317G/A and a second substitution selected from the group of substitutions consisting of T213G/V/A, L69V/Y/W/F, V254L/A/V/G, A209G, A152F/W/Y/L/V, L155T/W/F/V/L, F310G/A/L, and L318G/A/F, as determined with reference to SEQ ID NO:1, wherein when the cytochrome P450 comprises the substitution T213A, the cytochrome P450 additionally comprises a third substitution selected from said group of substitutions.