Systems and methods for an absorbance detector with optical reference
AssigneePhoseon Technology, Inc.
InventorsLowell Brunson, John Christopher Freitag, Theresa Thompson
Life of this patent
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Abstract
Systems and methods are provided for a UV-VIS spectrophotometer, such as a UV-VIS detector unit included in a high-performance liquid chromatography system. In one example, a system for the UV-VIS detector unit may include a first light source, a signal detector, a flow path positioned intermediate the first light source and the signal detector, a second light source, and a reference detector. The first light source, the signal detector, and the flow path may be aligned along a first axis, and the second light source and the reference detector may be aligned along a second axis, different than the first axis.
Claims17
A system, comprising:
- a first light source;
- a first signal detector;
- a first flow path positioned intermediate the first light source and the first signal detector, where the first flow path, the first light source, and the first signal detector are aligned along a first axis;
- a second light source;
- a second signal detector;
- a second flow path positioned intermediate the second light source and the second signal detector, where the second flow path, the second light source, and the second signal detector are aligned along a second axis, and the first flow path is fluidically coupled to the second flow path;
- and
- a controller including executable instructions stored in non-transitory memory that, when executed, cause the controller to:
- activate the first light source at a first activation time;
- and
- activate the second light source at a second activation time, wherein the second activation time is determined based on a flow rate of a sample from the first flow path to the second flow path.
A light detector system, comprising:
- a light source comprising at least two light emitters;
- a signal detector;
- a flow path positioned intermediate the light source and the signal detector;
- and
- a controller including executable instructions stored in non-transitory memory that, when executed, cause the controller to:
- modulate one or more of the at least two light emitters based on parameters of the light detector system, including:
- during a first mode, activate a first light emitter of the at least two light emitters, maintain a second light emitter of the at least two light emitters deactivated, and determine a first sample transmittance signal based on an output from the signal detector;
- and
- during a second mode, activate the second light emitter, maintain the first light emitter deactivated, and determine a second sample transmittance signal based on the output from the signal detector.
A system, comprising:
- a first light source;
- a first signal detector;
- a first flow path positioned intermediate the first light source and the first signal detector;
- a second light source;
- a second signal detector;
- a second flow path positioned intermediate the second light source and the second signal detector, the first flow path fluidically coupled to the second flow path;
- and
- a controller including executable instructions stored in non-transitory memory that, when executed, cause the controller to:
- determine a first sample transmittance signal based on output from the first signal detector as a sample flows through the first flow path;
- calculate a first absorbance of the sample based on the first sample transmittance signal;
- determine a second sample transmittance signal based on output from the second signal detector as the sample flows through the second flow path;
- calculate a second absorbance of the sample based on the second sample transmittance signal;
- determine a reference transmittance signal based on output from the first signal detector as the sample flows through the second flow path, and not through the first flow path;
- and
- correct the second absorbance of the sample based on the reference transmittance signal.
Dependencies are inferred from the claim text.