Culture medium for detection of bacterium belonging to genus Listeria
AssigneeNissui Pharmaceutical Co., Ltd.
InventorsShuhei Hosokawa, Shingo Mizuochi, Mitsuaki Kashida
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Abstract
A medium containing (A) a chromogenic substance which develops a color when decomposed by β-glucosidase or a fluorogenic substance which develops a fluorescence when decomposed by β-glucosidase; (B) a chromogenic substance which develops a color when decomposed by phosphatidyl inositol-specific phospholipase C or a fluorogenic substance which develops a fluorescence when decomposed by phosphatidyl inositol-specific phospholipase C; and (C) a sugar that is useful for the detection of Listeria monocytogenes and/or Listeria ivanovii.
Claims6
A medium suitable to detect and differentiate Listeria monocytogenes and/or Listeria ivanovii from other bacteria of the genus Listeria, comprising:
- a first chromogenic substance which develops a color when decomposed by β-glucosidase, or a first fluorogenic substance which develops a fluorescence when decomposed by β-glucosidase;
- a second chromogenic substance which develops a color when decomposed by phosphatidyl inositol-specific phospholipase C, or a second fluorogenic substance which develops a fluorescence when decomposed by phosphatidyl inositol-specific phospholipase C;
- and
- a sugar at a concentration of 5 g/L to 30 g/L at the time of detecting and differentiating Listeria monocytogenes and/or Listeria ivanovii from other bacteria of the genus Listeria.
Dependencies are inferred from the claim text.