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US 11,512,328 B2Granted
Methods and systems for 1-butanol production
AssigneeBiocheminsights, Inc.
InventorsDavid R. Dodds, William B. Armiger, Mattheos Koffas
Life of this patent
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Abstract
A combination of an electrochemical device for delivering reducing equivalents to a cell, and engineered metabolic pathways within the cell capable of utilizing the electrochemically provided reducing equivalents is disclosed. Such a combination allows the production of commodity chemicals by fermentation to proceed with increased carbon efficiency.
Claims15
A system for 1-butanol production, comprising:
- an electrochemical bioreactor module for providing reducing equivalents;
- an engineered cell for receiving and using the reducing equivalents to produce 1-butanol, wherein the engineered cell comprises four exogenously introduced enzymes that are pyruvate:formate lyase (Pfl, EC 2.3.1.54), formaldehyde dehydrogenase (Fld, EC 1.2.1.46), hexulose-6-phosphate synthase (HPS, EC 4.1.2.43), and 6-phospho-3-hexuloisomerase (HPI, EC 5.3.1.27), and wherein in the engineered cell the endogenous pyruvate dehydrogenase (Pdh, EC 1.2.4.1) has been disabled, deleted or otherwise rendered non-functional;
- wherein the four exogenously introduced enzymes convert pyruvate into formate without producing CO2, and convert the formate to fructose-6-phosphate;
- wherein the engineered cell is selected from a bacterium or a fungus.
1 more element
Dependencies are inferred from the claim text.