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US 11,512,318 B2Granted
Method for producing 3-fucosyllactose using Corynebacterium glutamicum
AssigneesSeoul National University R&Db Foundation, Advanced Protein Technologies Corp.
InventorsJin-Ho Seo, Sang-Min Jung, Do-Haeng Lee, Hyeong-Do Jeon
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Abstract
Disclosed is a method for producing 3-fucosyllactose using a wild Corynebacterium glutamicum strain. In addition, using the Corynebacterium glutamicum strain, which is a GRAS strain, 3-fucosyllactose can be produced at a high concentration, high yield and high productivity.
Claims4
A method of producing 3-fucosyllactose comprising
- culturing in a medium supplemented with lactose a recombinant Corynebacterium glutamicum transformed to express α-1, 3-fucosyltransferase, transformed to express GDP-D-mannose-4, 6-dehydratase, transformed to express GDP-L-fucose synthase, and transformed to express lactose permease, and
- isolating 3-fucosyllactose from the medium,
- wherein the recombinant Corynebacterium glutamicum has phosphomannomutase and GTP-mannose-1-phosphate guanylyltransferase.
Dependencies are inferred from the claim text.