Loading patent…
AssigneeEnzymaster (Ningbo) Bio-Engineering Co., Ltd
InventorsHaibin Chen, Yong Koy Bong, Qing Xu, Ameng Zhou, Tianran Shen, Jiadong Yang, Zhuhong Yang
Estimating the term…
The present invention provides engineered polypeptides that are useful for the asymmetric synthesis of β-hydroxy-α-amino acids under industrial-relevant conditions. The engineered polypeptides disclosed in this invention were developed through directed evolution based on the ability of catalytic synthesis of (2S, 3R)-2-amino-3-hydroxy-3-(4-nitrophenyl) propanoic acid. The present disclosure also provides polynucleotides encoding engineered polypeptides, host cells capable of expressing engineered polypeptides, and methods of producing β-hydroxy-α-amino acids using engineered polypeptides. Compared to other processes of preparation, the use of the engineered polypeptides of the present invention for the preparation of β-hydroxy-α-amino acids results in high purity of the desired stereoisomers, mild reaction conditions, low pollution and low energy consumption. So, it has good industrial application prospects.
An engineered aldolase polypeptide that condenses p-nitrobenzaldehyde with glycine to produce (2S, 3R)-2-amino-3-hydroxy-3-(4-nitrophenyl) propanoic acid, said engineered aldolase polypeptide comprising an amino acid sequence having at least one amino acid substitution relative to SEQ ID NO:2, said at least one substitution selected from the group consisting of L45I, P91H, and combinations thereof, further wherein said amino acid sequence is selected from the group consisting of SEQ ID NOs:
An engineered polypeptide, which is a polypeptide having aldolase activity, which comprises an amino acid sequence having at least 85% sequence identity to one of the amino acid sequences set forth in of SEQ ID NOs:
Dependencies are inferred from the claim text.