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US 11,512,300 B2Granted
Xanthan lyase variants and polynucleotides encoding same
AssigneeNovozymes A/S
InventorsJens Erik Nielsen, Allan Svendsen, Lars Anderson, Rune Nygaard Monrad, Rajendra Kulothungan Sainathan, Pernille F. Jensen, Kasper D. Rand, Geetha Hiremath Mendez, Sohel Dalal, Shilpi Agarwal
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Abstract
The present invention relates to xanthan lyase variants and methods for obtaining xanthan lyase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
Claims21
A xanthan lyase variant, which
- (a) comprises a substitution at one or more positions selected from the group consisting of G753, S754, S757, A769, L775 and D801, wherein the numbering corresponds to SEQ ID NO:
- 2, and wherein
- (i) the substitution at position 753 is with Ala, Arg, Asn, Asp, Cys, Gln, Glu, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val;
- (ii) the substitution at position 754 is with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Thr, Trp, Tyr, or Val
- (iii) the substitution at position 757 is with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Thr, Trp, Tyr, or Val,
- (iv) the substitution at position 769 is with Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val,
- (v) the substitution at position 775 is with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, and
- (vi) the substitution at position 801 is with Ala, Arg, Asn, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val;
- (b) has at least 85% and less than 100% sequence identity to SEQ ID NO:
- 2;
- and
- (c) has xanthan lyase activity.
9 more elements
Dependencies are inferred from the claim text.