Microfluidic apparatus, and system and method for introducing substance into cell

A method of introducing a substance into a cell, applied to a system for introducing a substance into a cell, comprising:

• the microfluidic apparatus comprising a cavity channel, a bulk wave generating device and a surface acoustic wave generating device;
• wherein a microstructure is arranged on an inner wall of the cavity channel, and the microstructure is constructed for forming a bubble by a solution at the microstructure in a case where the solution is injected into the cavity channel;
• the bulk wave generating device is configured to generate a bulk wave, wherein the bulk wave enables the bubble to resonate for generating a flow field;
• and
• the surface acoustic wave generating device is configured to generate a surface acoustic wave and control a position of at least one particle in the solution;
• and
• an injection device, which is connected to an inlet of the cavity channel of the microfluidic apparatus, wherein the injection device is configured to inject a solution containing the cell into the cavity channel of the microfluidic apparatus, and the bubble is generated by the solution at the microstructure of the cavity channel;
• wherein the microfluidic apparatus is configured to:
• generate the bulk wave, wherein the bulk wave enables the bubble to resonate, causes vibration of the solution in the cavity channel, and generates a flow field in the cavity channel, wherein each position in the flow field corresponds to a different shear stress;
• and
• control a position of the cell in the flow field relative to the microstructure to enable the cell to flow through a preset position in a case where the cell flows in the flow field, wherein a reversible through hole is formed on the cell under an action of a shear stress corresponding to the preset position, and the substance in the solution enters the cell through the reversible through hole, the method comprising the steps of:
• injecting a solution containing the cell into a cavity channel of a microfluidic apparatus, wherein the solution containing the cell generates a bubble at a microstructure of the cavity channel;
• using a bulk wave generating device of the microfluidic apparatus to generate a bulk wave, wherein the bulk wave enables the bubbles to resonate, causes vibration of the solution in the cavity channel, and generates a first flow field in the cavity channel, wherein each position in the first flow field corresponds to a different shear stress;
• and
• using a surface acoustic wave generating device of the microfluidic apparatus to control a position of the cell in the first flow field relative to the microstructure for enabling the cell to flow through a preset position in a case where the cell flows in the first flow field, wherein the cell generates a reversible through hole under an action of a shear stress corresponding to the preset position, and the substance in the solution enters the cell through the reversible through hole.