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AssigneesInserm (Institut National De La Sante Et De La Recherche Medicale), Université Paris Cité
InventorsCatherine Le Visage, Didier Letourneur
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The present invention relates to a method for preparing a porous scaffold for tissue engineering. It is another object of the present invention to provide a porous scaffold obtainable by the method as above described, and its use for tissue engineering, cell culture and cell delivery. The method of the invention comprises the steps consisting of: a) preparing an alkaline aqueous solution comprising an amount of at least one polysaccharide, an amount of a cross-linking agent and an amount of a porogen agent b) transforming the solution into a hydrogel by placing said solution at a temperature from about 4° C. to about 80° C. for a sufficient time to allow the cross-linking of said amount of polysaccharide and c) submerging said hydrogel into an aqueous solution d) washing the porous scaffold obtained at step c).
A porous scaffold comprising a polysaccharide selected from the group consisting of dextran, agar, pullulan, inulin, heparin, fucoidan, and mixtures thereof, crosslinked with trisodium trimetaphosphate, wherein the average pore size of the scaffold is between 1 and 500 μm, wherein the scaffold comprises pores of different sizes, and wherein the porosity is in the range from 4% to 50%, and wherein pores in the scaffold have an elongated shape, and are formed using a carbonate salt selected from the group consisting of ammonium carbonate, ammonium bicarbonate, sodium carbonate, sodium bicarbonate, calcium carbonate, and mixtures thereof and freeze drying, and wherein the pores are not formed by using a surfactant.
Dependencies are inferred from the claim text.