Ecosystem device for determining plant-microbe interactions

A device comprising:

• a base;
• a substrate in contact with a first surface of the base, the substrate and the base defining a root chamber;
• and
• an enclosure in contact with a second surface of the base, the base and the enclosure defining a growth chamber, the base defining a stem port connecting the root chamber and the growth chamber, the base further defining a first port in fluid communication with the root chamber and a second port in fluid communication with the root chamber, the device being operable to contain a plant, roots of the plant being in the root chamber, a stem of the plant passing through the stem port, and leaves of the plant being in the growth chamber, the base further defining a raised platform on a surface of the base proximate the second port, the raised platform and the substrate defining a channel being operable to permit fluid to flow from the root chamber to the second port, the raised platform being operable to prevent roots of the plant from growing into the second port.