Methods and materials for producing 7-carbon monomers
AssigneeINV Nylon Chemicals Americas, LLC
InventorsAlex Van Eck Conradie, Adriana Leonora Botes, Jonathan Kennedy, Nadia Fatma Kadi
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Abstract
This document describes biochemical pathways for producing 7-aminoheptanoic acid using a β-ketoacyl synthase or a β-ketothiolase to form an N-acetyl-5-amino-3-oxopentanoyl-CoA intermediate. 7-aminoheptanoic acid can be enzymatically converted to pimelic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol or corresponding salts thereof. This document also describes recombinant microorganisms producing 7-aminoheptanoic acid as well as pimelic acid, 7-hydroxyheptanoic acid, heptamethylenediamine and 1,7-heptanediol or corresponding salts thereof.
Claims24
A method of producing N-acetyl-5-amino-3-oxopentanoyl-CoA or a salt thereof, said method comprising enzymatically converting N-acetyl-3-aminopropanoyl-CoA to N-acetyl-5-amino-3-oxopentanoyl-CoA or a salt thereof using a polypeptide having the activity of a β-ketoacyl synthase or a β-ketothiolase classified under EC. 2.3.1.- and/or a CoA transferase classified under EC 2.8.3.-,
- wherein said polypeptide having the activity of a β-ketothiolase has at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NOs:
- 1 or 13,
- said polypeptide having the activity of a β-ketoacyl synthase has at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO:
- 14, and
- said polypeptide having the activity of a CoA transferase has at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO:
- 19.
Dependencies are inferred from the claim text.