Hybrid polyketide synthases
AssigneeThe Regents of the University of California
InventorsJeffrey L. Fortman, Andrew Hagen, Leonard Katz, Jay D. Keasling, Sean Poust, Jingwei Zhang, Sergey Zotchev
Life of this patent
Estimating the term…
Abstract
The present invention provides for a polyketide synthase (PKS) capable of synthesizing an even-chain or odd-chain diacid or lactam or diamine. The present invention also provides for a host cell comprising the PKS and when cultured produces the even-chain diacid, odd-chain diacid, or KAPA. The present invention also provides for a host cell comprising the PKS capable of synthesizing a pimelic acid or KAPA, and when cultured produces biotin.
Claims11
At least one recombinant nucleic acid encoding-a non-naturally occurring polyketide synthase (PKS) that synthesizes pimelic acid, wherein the PKS is a hybrid PKS comprising (a) a loading module that loads malonate comprising a malonyl-specific AT domain linked to an acyl carrier protein (ACP) domain, wherein the ACP domain is linked to (b) an extender module comprising a ketosynthase (KS) domain, an acytransferase (AT) domain linked to a dehydratase (DH) domain linked to an enoyl reductase (ER) domain linked to a ketoreductase (KR) domain linked to an acyl carrier protein (ACP) domain;
- and (c) a further extender modulus, that extends an extended product of (b), comprising a KS domain linked to an AT domain linked to a DH domain linked to a KR domain linked to an ACP domain, wherein the extender module of (b) and the further extender molecule of (c) each extend with malonate;
- and wherein the extender module of (c) comprises a hydrolytic thioesterase (TE) domain at the C-terminus.
Dependencies are inferred from the claim text.