Method for co-culturing Inonotus obliquus, Ganoderma lucidum, and Phellinus linteus mycelia
AssigneeGiunchan Co., Ltd.
InventorJong-Yea Park
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Abstract
The present invention relates to a method of co-culturing Inonotus obliquus, Ganoderma lucidum, and Phellinus linteus. The co-cultured mycelia prepared through the method of the present invention have high beta-glucan content and thus can exhibit superior health functionality, and can be used as an additive or a cooking seasoning in various foods. In addition, the use of the co-cultured mycelia in curing raw meat enables easy preparation of a meat-based food product that has a good taste and flavor.
Claims8
A method of co-culturing Inonotus obliquus, Ganoderma lucidum and Phellinus linteus mycelia, comprising the following steps in order:
- (1) inoculating a fruit body tissue of each of Inonotus obliquus, Gano derma lucidum and Phellinus linteus in Potato Dextrose Agar (PDA) and then separately culturing mycelia of each of the Inonotus obliquus, Gano derma lucidum and Phellinus linteus;
- (2) co-inoculating the mycelia of the Inonotus obliquus, Ganoderma lucidum and Phellinus linteus separately cultured in the step (1) in Potato Dextrose Broth (PDB);
- (3) culturing the PDB inoculated with the mycelia of the Inonotus obliquus, Ganoderma lucidum and Phellinus linteus for 4-6 weeks;
- (4) inoculating the mycelia obtained through the culturing in the step (3) in a rice barley medium;
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- (5) further culturing the mycelia inoculated in the rice barley medium in the step (4) for 4-7 weeks to obtain co-cultured mycelia.
Dependencies are inferred from the claim text.