US 11,499,189 B2 · Mitigating adverse impacts of detection systems on nucleic acids and other biological analytes

Abstract

A method for sequencing a population of nucleic acids, which includes (a) binding the population of nucleic acids with a fractionally labeled mixture of nucleotides, thereby forming a fractionally labeled population of nucleic acids, wherein the mixture includes nucleotide cognates for a common base type in the templates, and wherein a fraction of the nucleotide cognates for the common base type in the mixture are exogenously labeled nucleotides that produce a signal that is not produced by other nucleotide cognates for the common base type in the mixture; (b) detecting the signal from the fractionally labeled population of nucleic acids; and (c) repeating (a) and (b) using a second mixture of the fractionally labeled nucleotides, wherein the fraction of the exogenously labeled nucleotides is higher in the second mixture.

Claims20

Dependent claims

A method for sequencing a population of nucleic acids, comprising

subjecting a population of primer-template nucleic acid hybrids to multiple cycles of a nucleic acid sequencing process, wherein the templates share a common sequence, wherein each of the cycles comprises:
(a) forming a mixed population comprising primer-template nucleic acid hybrids that are bound to a first nucleotide type and primer-template nucleic acid hybrids that are bound to a second nucleotide type, wherein the first nucleotide type comprises an exogenous label that produces a first signal and wherein the second nucleotide type is substantially devoid of labels that produce the first signal, wherein the first and second nucleotide types are cognates for the same base type in the template, and
(b) detecting the signal from the mixed population,
wherein the fraction of the first nucleotide type is increased in the mixed population over at least two of the cycles of the nucleic acid sequencing process.

1 more element

Dependencies are inferred from the claim text.

Mitigating adverse impacts of detection systems on nucleic acids and other biological analytes

Life of this patent

Mitigating adverse impacts of detection systems on nucleic acids and other biological analytes

Life of this patent

Abstract

Claims20