Mutant type 2-deoxy-scyllo-inosose synthase
AssigneeMitsui Chemicals, Inc.
InventorsHiroaki Takaku, Harutake Yamazaki, Mitsufumi Wada, Daisuke Miyazawa
Life of this patent
Estimating the term…
Abstract
A polypeptide includes, in the amino acid sequence of SEQ ID NO: 1 or a similar sequence, at least one specific amino acid substitution on at least one of the 14th, 37th, 209th, 293rd, and 319th amino acid residues from the N-terminal of the amino acid sequence of SEQ ID NO: 1. A polynucleotide, an expression cassette, a vector, and a transformant include a base sequence encoding the amino acid sequence of the polypeptide. A method of producing the polypeptide and a method of producing 2-deoxy-scyllo-inosose are also provided.
Claims13
A polypeptide having 2-deoxy-scyllo-inosose (DOI) synthesis activity, wherein the polypeptide comprises an amino acid sequence that has at least 95% sequence identity to the amino acid sequence of SEQ ID NO:
- 1, and has an amino acid substitution selected from the group consisting of:
- (a) the asparagine corresponding to position 14 of SEQ ID NO:
- 1 is substituted with threonine;
- (b) the tyrosine corresponding to position 37 of SEQ ID NO:
- 1 is substituted with phenylalanine;
- (c) the alanine corresponding to position 290 of SEQ ID NO:
- 1 is substituted with threonine;
- (d) the histidine corresponding to position 319 of SEQ ID NO:
- 1 is substituted with arginine;
- and
- (e) a combination of any of the substitutions (a), (b), (c), and (d),
- wherein the polypeptide has a higher DOI synthesis activity than a wild-type DOI synthase having the amino acid sequence of SEQ ID NO:
- 1.
A polypeptide having DOI synthesis activity, wherein the polypeptide comprises an amino acid sequence that has at least 80% sequence identity to the amino acid sequence of SEQ ID NO:
- 1, and has an amino acid substitution selected from the group consisting of:
- (a) the asparagine corresponding to position 14 of SEQ ID NO:
- 1 is substituted with threonine;
- (b) the tyrosine corresponding to position 37 of SEQ ID NO:
- 1 is substituted with phenylalanine;
- (c) the histidine corresponding to position 319 of SEQ ID NO:
- 1 is substituted with arginine;
- and
- (d) a combination of any of the substitutions (a), (b), and (c),
- wherein the polypeptide has a higher DOI synthesis activity than a wild-type DOI synthase having the amino acid sequence of SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.