US 11,499,171 B2 · Method of producing 13-hydroxy-9(Z)-octadecenoic acid

Abstract

The present invention provides a method of producing 13-hydroxy-9(Z)-octadecenoic acid, productivity of which has been enhanced. Specifically, the present invention provides a method of producing 13-hydroxy-9(Z)-octadecenoic acid, by producing 13-hydroxy-9(Z)-octadecenoic acid from linoleic acid in the presence of a transformed microorganism that produces a protein such as the following: (A) a protein having an amino acid sequence of SEQ ID NOs: 4, 5, 8 to 10, 13, or 14; (B) a protein having an amino acid sequence containing one or several amino acid substitutions, deletions, insertions or additions in the amino acid sequence of SEQ ID NOs: 4, 5, 8 to 10, 13, or 14, and having a linoleate 13-hydratase activity; and (C) a protein having an amino acid sequence having 90% or more identity to the amino acid sequence of SEQ ID NOs: 4, 5, 8 to 10, 13 or 14, and having a linoleate 13-hydratase activity.

A method of producing 13-hydroxy-9(Z)-octadecenoic acid, comprising producing 13-hydroxy-9(Z)-octadecenoic acid from linoleic acid in the presence of a transformed microorganism that produces a protein selected from the group consisting of:

(1A) a protein comprising the amino acid sequence of SEQ ID NO:
4;
(1B) a protein comprising an amino acid sequence containing one to twenty amino acid substitutions, deletions, insertions or additions the amino acid sequence of SEQ ID NO:
4, and having a linoleate 13-hydratase activity;
(1C) a protein comprising an amino acid sequence having 95% or more identity to the amino acid sequence of SEQ ID NO:
4, and having a linoleate 13-hydratase activity;
(2A) a protein comprising the amino acid sequence of SEQ ID NO:
5;
(2B) a protein comprising an amino acid sequence containing one to twenty amino acid substitutions, deletions, insertions or additions the amino acid sequence of SEQ ID NO:
5, and having a linoleate 13-hydratase activity;
(2C) a protein comprising an amino acid sequence having 95% or more identity to the amino acid sequence of SEQ ID NO:
5, and having a linoleate 13-hydratase activity;
(3A) a protein comprising the amino acid sequence of SEQ ID NO:
8;
(3B) a protein comprising an amino acid sequence containing one to fifty amino acid substitutions, deletions, insertions or additions the amino acid sequence of SEQ ID NO:
8, and having a linoleate 13-hydratase activity;
(3C) a protein comprising an amino acid sequence having 90% or more identity to the amino acid sequence of SEQ ID NO:
8, and having a linoleate 13-hydratase activity;
(4A) a protein comprising the amino acid sequence of SEQ ID NO:
9;
(4B) a protein comprising an amino acid sequence containing one to fifty amino acid substitutions, deletions, insertions or additions the amino acid sequence of SEQ ID NO:
9, and having a linoleate 13-hydratase activity;
(4C) a protein comprising an amino acid sequence having 90% or more identity to the amino acid sequence of SEQ ID NO:
9, and having a linoleate 13-hydratase activity;
(5A) a protein comprising the amino acid sequence of SEQ ID NO:
10;
(5B) a protein comprising an amino acid sequence containing one to fifty amino acid substitutions, deletions, insertions or additions the amino acid sequence of SEQ ID NO:
10, and having a linoleate 13-hydratase activity;
(5C) a protein comprising an amino acid sequence having 90% or more identity to the amino acid sequence of SEQ ID NO:
10, and having a linoleate 13-hydratase activity;
(6A) a protein comprising the amino acid sequence of SEQ ID NO:
13;
(6B) a protein comprising an amino acid sequence containing one to fifty amino acid substitutions, deletions, insertions or additions the amino acid sequence of SEQ ID NO:
13, and having a linoleate 13-hydratase activity;
(6C) a protein comprising an amino acid sequence having 90% or more identity to the amino acid sequence of SEQ ID NO:
13, and having a linoleate 13-hydratase activity;
(7A) a protein comprising the amino acid sequence of SEQ ID NO:
14;
(7B) a protein comprising an amino acid sequence containing one to fifty amino acid substitutions, deletions, insertions or additions the amino acid sequence of SEQ ID NO:
14, and having a linoleate 13-hydratase activity;
and
(7C) a protein comprising an amino acid sequence having 90% or more identity to the amino acid sequence of SEQ ID NO:
14, and having a linoleate 13-hydratase activity.

40 more elements

Method of producing 13-hydroxy-9(Z)-octadecenoic acid

Life of this patent

Method of producing 13-hydroxy-9(Z)-octadecenoic acid

Life of this patent

Abstract

Claims20