Transgenic plant with reduced fucosyltransferase and xylosyltransferase activity
AssigneePlantForm Corporation
InventorsMichael D. McLean, Zacharie LeBlanc
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Abstract
A genetically modified plant or plant cell with reduced α1,3-fucosyltransferase and β1,2-xylosyltransferase activity compared to a wild type plant or plant cell, wherein less than 10% of the total glycan on a protein produced by the plant or plant cell is α1,3-fucosylated glycan and less than 3% of the total glycan on the protein is β1,2-xylosylated glycan is provided. In one embodiment, the plant or plant cell comprises three T-DNA insertions expressing five copies of RNAi targeting α1,3-fucosyltranserase and three copies of RNAi targeting β1,2xylosyltransferase.
Claims5
A genetically modified Nicotiana benthamiana plant, plant part or plant cell wherein the plant, plant part or plant cell comprises
- (a) a first T-DNA insertion consisting of the reverse complement of SEQ ID NO:
- 15;
- (b) a second T-DNA insertion consisting of SEQ ID NO:
- 16;
- and
- (c) a third T-DNA insertion consisting of the reverse complement of SEQ ID NO:
- 17,
- wherein seeds comprising said first, second, and third T-DNA insertions have been deposited at the ATCC under Accession No. PTA-127135.
Dependencies are inferred from the claim text.