Nucleic acid encoding hexuronate C4-epimerase variant having improved D-tagatose conversion activity, and D-tagatose production method using same
AssigneeCj Cheiljedang Corporation
InventorsSung Jae Yang, Young Mi Lee, Il Hyang Park, Chan-Hyoung Lee, Hyun Kug Cho, Seong Bo Kim, Yang Hee Kim, Seung Won Park
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Abstract
Provided are a hexuronate C4-epimerase variant with improved activity in converting D-fructose by D-tagatose of hexuronate C4-epimerase and a method for production of D-tagatose using them.
Claims9
A nucleic acid encoding a hexuronate C4-epimerase variant in which serine (S) as an amino acid residue at position 185 from the N-terminus of a hexuronate C4-epimerase consisting of the amino acid sequence set forth in SEQ ID NO:
- 1 is replaced by alanine (A), glycine (G), histidine (H), lysine (K), glutamine (Q) or arginine (R).
A method for D-tagatose production, comprising bringing a hexuronate C4-epimerase variant in which serine (S) as an amino acid residue at position 185 from the N-terminus of a hexuronate C4-epimerase consisting of the amino acid sequence set forth in SEQ ID NO:
- 1 is replaced by alanine (A), glycine (G), histidine (H), lysine (K), glutamine (Q) or arginine (R).
Dependencies are inferred from the claim text.