Cell treatment apparatus and method for treating object to be treated

A method for treating an object to be treated, the method comprising:

• cutting an object to be treated that includes a light-absorbing layer and a culture existing above the light-absorbing layer by irradiating a region of the light-absorbing layer corresponding to a region to be cut in the culture with light to cut the culture existing above the light-absorbing layer into a predetermined shape;
• and
• treating the light-absorbing layer corresponding to a portion other than the region to be cut by irradiating with light to subject the culture existing above the light-absorbing layer to a killing treatment, wherein
• a first spot diameter formed in a portion to be irradiated with the light in the light-absorbing layer by the light in the cutting step is different from a second spot diameter formed in a portion to be irradiated in the light-absorbing layer by the light in the treating step;
• wherein the method is implemented by a cell treatment apparatus that comprises:
• a first region;
• and
• a second region, wherein
• the first region and the second region are placed in succession,
• the first region is a cell treatment chamber for treating cells,
• the cell treatment chamber is closeable from outside of the cell treatment chamber and comprises a culture vessel placement portion for placing a cell culture vessel,
• the second region comprises:
• a laser irradiation device;
• an imaging device;
• and
• a spot diameter adjustment device,
• wherein
• the laser irradiation device comprises a laser light source and a laser emitter, and the laser irradiation device is capable of irradiating the cell culture vessel placed in the culture vessel placement portion with a laser emitted via the laser emitter,
• the imaging device comprises objective lenses,
• the spot diameter adjustment device adjusts a spot diameter formed in a portion to be irradiated with the laser in an object to be irradiated,
• the culture vessel placement portion is placed to be adjacent to the second region in the cell treatment chamber,
• an adjacent portion to the second region in the culture vessel placement portion is translucent, and
• electric power to the laser irradiation device and the imaging device is supplied from outside of the first region and the second region.