Immobilised biological entities
AssigneeW. L. Gore & Associates, Inc.
InventorsKarin Leontein, Per Antoni, Daniel Nyström, Paul Begovac, Krzysztof Pietrzak
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Abstract
There is described inter alia a device having a surface comprising a layered coating wherein the outer coating layer comprises a plurality of cationic hyperbranched polymer molecules characterized by having (i) a core moiety of molecular weight 14-1,000 Da (ii) a total molecular weight of 1,500 to 1,000,000 Da (iii) a ratio of total molecular weight to core moiety molecular weight of at least 80:1 and (iv) functional end groups, whereby one or more of said functional end groups have an anti-coagulant entity covalently attached thereto.
Claims20
A non-thrombogenic device having a surface comprising a layered coating comprising an outer coating layer and an inner coating layer, wherein the outer coating layer comprises a plurality of cationic hyperbranched polymer molecules characterized by having:
- (i) a core moiety having a core moiety molecular weight of 14 to 1,000 Da;
- (ii) a total molecular weight of 1,500 to 1,000,000 Da;
- (iii) a ratio of the total molecular weight to the core moiety molecular weight of at least 80:1;
- and
- (iv) a plurality of functional end groups, whereby one or more of said functional end groups have an anti-coagulant entity covalently attached thereto,
- wherein the anti-coagulant entity is a heparin moiety,
- which is prepared by a process comprising, in order:
- (a) reacting a plurality of functional end groups of the hyperbranched polymer molecules with anti-coagulant entities such that each hyperbranched polymer molecule is covalently linked to a plurality of anti-coagulant entities;
- (b) attaching the hyperbranched polymer molecules to the outer coating layer;
- and
- (c) subjecting the device to ethylene oxide sterilization;
- wherein step (a) is carried out in solution,
- wherein the hyperbranched polymer is a dendrimer.
A non-thrombogenic device having a surface comprising a layered coating comprising an outer coating layer and an inner coating layer, wherein the outer coating layer comprises a plurality of cationic hyperbranched polymer molecules characterized by having:
- (i) a core moiety having a core moiety molecular weight of 14 to 1,000 Da;
- (ii) a total molecular weight of 1,500 to 1,000,000 Da;
- (iii) a ratio of the total molecular weight to the core moiety molecular weight of at least 80:1;
- and
- (iv) a plurality of functional end groups, whereby one or more of said functional end groups have an anti-coagulant entity covalently attached thereto,
- wherein the anti-coagulant entity is a heparin moiety,
- which is prepared by a process comprising, in order:
- (a) reacting a plurality of functional end groups of the hyperbranched polymer molecules with anti-coagulant entities such that each hyperbranched polymer molecule is covalently linked to a plurality of anti-coagulant entities;
- (b) attaching the hyperbranched polymer molecules to the outer coating layer;
- and
- (c) subjecting the device to ethylene oxide sterilization;
- wherein step (a) is carried out in solution,
- wherein the hyperbranched polymer is selected from polyamidoamine, polypropyleneimine, polyethyleneimine and polyaminepolymers and copolymers comprising one or more of polyamidoamine, polypropyleneimine, polyethyleneimine and polyamine hyperbranched polymers.
Dependencies are inferred from the claim text.