Zoonotic disease RNA vaccines

A composition comprising:

• (a) a messenger ribonucleic acid (mRNA) comprising an open reading frame (ORF) encoding a fusion protein, wherein the fusion protein comprises a Nipah virus F protein and a Nipah virus G protein;
• and
• (b) a lipid nanoparticle,
• wherein the lipid nanoparticle comprises 45-55 mol % ionizable lipid, 5-25 mol % neutral lipid, 35-45 mol % sterol, and 0.5-5 mol % polyethylene glycol (PEG)-modified lipid,
• wherein the ionizable lipid is a compound of Formula (I):
• and wherein
• R1 is R″M′R′ or C5-20 alkenyl;
• R2 and R3 are each independently selected from C1-14 alkyl and C2-14 alkenyl;
• R4 is —(CH2)nQ, wherein Q is OH and n is selected from 3, 4, and 5;
• M and M′ are each independently —OC(O)— or —C(O)O—;
• R5, R6, and R7 are each H;
• R′ is a linear C1-12 alkyl, or C1-12 alkyl substituted with C6-9 alkyl;
• R″ is C3-14 alkyl;
• m is selected from 5, 6, 7, 8, 9, 10, 11, 12, and 13.