Virus-like particle with efficient epitope display

A host cell expressing:

• i. a first polynucleotide encoding a virus capsid protein comprising a first peptide tag;
• and/or
• ii. a second polynucleotide encoding an antigen fused to a second peptide tag,
• wherein the antigen and virus capsid protein are linked via an isopeptide bond between the first and second peptide tag, and wherein i-ii form a virus-like particle displaying said antigen.

A method for inducing an immune response in a subject, the method comprising the steps of:

• a. obtaining a composition comprising:
• i) a virus capsid protein comprising a first peptide tag, and
• ii) an antigen fused to a second peptide tag,
• wherein the antigen and virus capsid protein are linked via an isopeptide bond between the first and second peptide tag, and wherein i-ii form a virus-like particle displaying said antigen, and
• b. administering said composition to a subject at least once for prophylaxis and/or treatment of a disease,
• thereby inducing an immune response in the subject.