Cell cryopreservation protective composition and use thereof

A cell cryopreservation protective composition comprising one or more zwitterionic molecules having a structure of general formula R1—N+(CH3)2—(CH2)n—R2, and a nutrient ingredient for cells,

• wherein said zwitterionic molecules are 10 to 2530 parts by mass with respect to 100 parts by mass of said nutrient ingredient for cells;
• said cell cryopreservation protective composition excludes the following substances:
• glycerol, diaminoethane tetraacetic acid or salts thereof, dimethyl sulfoxide, or yolk,
• in said structure of general formula R1—N+(CH3)2—(CH2)n—R2,
• said R1 is linear or branched alkyl having 1 to 10 carbon atoms, and is optionally substituted with a substituent selected from the group consisting of (meth)acryloylamino, (meth)acryloyloxy, alkenyl, hydroxyl, hydroxyalkyl, alkoxyl, and halogen,
• said R2 is any negatively charged group selected from the group consisting of —COO−, —SO4−, —SO3−, and
• and said R3 is a group selected from the group consisting of (meth)acryloyloxyalkyl, alkyl, and alkenyl,
• said (CH3)2 and (CH2)n may each independently be optionally substituted with a substituent selected from the group consisting of alkyl, alkenyl, hydroxyl, hydroxyalkyl, alkoxyl, a polyalkylene oxide group, and halogen, and
• n is an integer from 1 to 10,
• wherein said nutrient ingredient for cells is selected from the group consisting of one or more of the amino acids arginine, glycine, leucine, glutamine, glutamic acid, isoleucine, and proline.