Coated substrate for biological reaction systems
AssigneeLife Technologies Corporation
InventorsEliodor Ghenciu, Theodore E. Straub, Evan W. Foster, Michael C. Pallas
Life of this patent
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Abstract
An apparatus for biological reactions is provided. The apparatus includes a substrate and a plurality of reaction sites within the substrate. A surface of the substrate is configured to have a first hydrophilicity and each surface of the plurality of reaction sites is configured to have a second hydrophilicity to load a substantial number of reaction sites with a sample volume. The sample volume of each loaded reaction site is substantially confined to its respective reaction site. The sample volume is configured to undergo a biological reaction within the reaction site.
Claims14
An apparatus for biological reactions, the apparatus comprising:
- a substrate comprising a substrate surface;
- a plurality of reaction sites within the substrate, wherein each reaction site comprises a reaction site surface;
- and
- a coating material, wherein the coating material coats the substrate surface and each reaction site surface, the coated substrate surface has a water contact angle of 60-100 degrees, the coated substrate surface has an advancing contact angle of 70-85 degrees, and each of the coated reaction site surfaces is configured to load the reaction sites with a sample for undergoing a biological reaction.
An instrument, comprising:
- a detection system;
- control system configured to control the functions of the detection system;
- a sample support device including substrate comprising a substrate surface and a plurality of reaction sites disposed along the substrate surface, each reaction site comprising a reaction site surface;
- a coating material, wherein:
- the coating material coats the substrate surface, to provide a coated substrate surface,
- the coating material coats each of the reaction site surfaces to provide a plurality of coated reaction sites,
- the coated substrate surface has a water contact angle of 60-100 degrees, and
- the coated substrate surface has an advancing contact angle of 70-85 degrees.
Dependencies are inferred from the claim text.