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US 11,493,446 B2Granted
Methods and compositions for assessing cell viability
OwnerKyle Gee, Kathleen Kihn +3inventors
InventorsKyle Gee, Kathleen Kihn, Yi-Zhen Hu, Yexin Wu, Kathleen Free
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Abstract
Provided herein are compounds, compositions, kits, uses, and methods for assessing the viability of cells and/or quantifying the amount of live and dead cells in a mixture using differential stains. In some embodiments, the compounds are used in culture media or can function independently of fixation and/or permeabilization. In some embodiments, the compounds comprise a platinum atom. In some embodiments, the compounds comprise a nitrogen mustard moiety.
Claims17
A compound of Formula I:
- D-L-R (I)
- wherein:
- D is a fluorescent moiety;
- L is a linker;
- and
- R is Formula IA:
- —B—N(R1)(R2) (Formula IA),
- wherein B is a bond, an aliphatic group or an aromatic group;
- and
- R1 and R2 are each independently ethyl substituted with a Z at the 2 position or substituted ethyl further substituted with a Z at the 2 position, and each Z is independently OH, Cl, Br, I or
- where R5 is alkyl, substituted alkyl, aryl or substituted aryl.
8 more elements
Dependencies are inferred from the claim text.