3′-UTR sequences for stabilization of RNA

An RNA molecule comprising in the 5′ 3′ direction:

• (a) a 5′-untranslated region;
• (b) a nucleic acid sequence coding for a peptide or protein;
• and
• (c) a 3′-untranslated region which is not naturally linked to the nucleic acid sequence of (b), said 3′-untranslated region comprising a nucleic acid sequence of the 3′-untranslated region of AES, which comprises:
• (A) a nucleic acid sequence selected from the group consisting of SEQ ID NOs:
• 86 to 89,
• (B) a fragment comprising at least 80% of any one of SEQ ID NOs:
• 86 to 89, or
• (C) a variant comprising a nucleic acid sequence that is at least 90% identical to the nucleic acid sequence (A) or the fragment (B).

A method of obtaining RNA, the method comprising:

• (A) coupling a nucleic acid sequence (b) which codes for a 3′-untranslated region to a transcribable nucleic acid sequence (a) comprising a nucleic acid sequence coding for a peptide or protein to form a nucleic acid molecule, and is characterized in that, when the nucleic acid molecule is transcribed, the nucleic acid sequence (b) is located at 3′ end of the transcribable nucleic acid sequence (a), and
• (B) transcribing the nucleic acid molecule from (A) to produce RNA comprising said 3′-untranslated region,
• wherein said 3′-untranslated region comprises (i) a nucleic acid sequence selected from the group consisting of SEQ ID NOs:
• 86 to 89, (ii) a fragment comprising at least 80% of any one of SEQ ID NOs:
• 86 to 89, or (iii) a variant comprising a nucleic acid sequence that is at least 90% identical to the nucleic acid sequence (i) or the fragment (ii).

A nucleic acid molecule comprising in the 5′ 3′ direction of transcription relative to synthesis of a transcript:

• (a) a promoter;
• (b) a transcribable nucleic acid sequence or a nucleic acid sequence for introducing a transcribable nucleic acid sequence;
• and
• (c) a nucleic acid sequence which codes for a 3′-untranslated region
• wherein the nucleic acid molecule is characterized in that so that, when the nucleic acid molecule is transcribed under control of the promoter (a), a transcript is produced that includes the 3′-untranslated region (c), wherein said 3′-untranslated region (c) is not naturally linked to the transcribable nucleic acid sequence (b),
• wherein said 3′-untranslated region in the transcript comprises a combination of a nucleic acid sequence of the 3′-untranslated region of Amino-Terminal Enhancer of Split (AES) and a nucleic acid sequence of the non-coding RNA of MT-RNR1, which combination has the nucleic acid sequence of SEQ ID NO:
• 174.

An RNA molecule comprising in the 5′ 3′ direction:

• (a) a 5′-untranslated region;
• (b) a nucleic acid sequence coding for a peptide or protein;
• and
• (c) a 3′-untranslated region which is not naturally linked to the nucleic acid sequence (b), said 3′-untranslated region comprising a combination of a nucleic acid sequence of the 3′-untranslated region of Amino-Terminal Enhancer of Split (AES) and a nucleic acid sequence of the non-coding RNA of MT-RNR1, which combination has the nucleic acid sequence of SEQ ID NO:
• 174.