Highly active S-cyanohydrin lyase and application thereof
AssigneeAbiochem Biotechnology Co., Ltd.
InventorsZhenhua Tian, Zhanbing Cheng, Chuanmin Sun
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Abstract
The invention provides a highly active S-cyanohydrin lyase obtained by mutating an amino acid residue at position 103 of a wild-type cassava S-cyanohydrin lyase. The mutation can significantly increase an expression of a mutant enzyme in E. coli and does not require a decrease in temperature when induced. Further mutations at position 128 and other sites were performed to obtain mutants with increased catalytic activity.
Claims8
A mutated S-cyanohydrin lyase, which is mutated at two or more sites comprising amino acid residue of position 103 and amino acid residue of position 128 of SEQ ID NO. 1, wherein the amino acid residue of position 103 is mutated from H to L, I, V, C, S or M, the amino acid residue of position 128 is mutated from W to A, N, L, V, G or Y, and the amino acid sequence of the mutated S-cyanohydrin lyase has at least 80% homology to SEQ ID NO. 1.
Dependencies are inferred from the claim text.