Holographic imaging device and method

A method of using a holographic imaging device, the method comprises:

• illuminating, by an imaging unit comprising at least two light sources, an object by emitting a first light beam with a first of the at least two light sources and a second light beam with a second of the at least two light sources, wherein a first light beam has a first wave-vector and a first wavelength and a second light beam has a second wave-vector that is different from the first wave-vector and a second wavelength that is different from the first wavelength,
• obtaining, by a processing unit, at least two holograms of the object by controlling the imaging unit to sequentially illuminate the object with respectively the first light beam and the second light beam,
• obtaining, by the processing unit, at least two phase-retrieved holograms based on estimating a phase of each of the at least two holograms,
• processing, by the processing unit, the at least two phase-retrieved holograms by:
• determining a signature for an out-of-focus plane signal originating from a ghost image of the object, based on a comparison of the at least two phase-retrieved holograms, and removing, from the at least two phase-retrieved holograms, at least one out-of-focus plane signal originating from the ghost image of the object,
• constructing, by the processing unit, at least two two-dimensional (2D) image slices based on the at least two processed phase-retrieved holograms, wherein each 2D image slice is constructed at a determined depth within an object volume of the object, and
• generating, by the processing unit, a three-dimensional (3D) image of the object based on a combination of the 2D image slices.