Cells and methods for production of lysine and lysine-derived products
AssigneesCathay Biotech Inc., Cibt America Inc.
InventorsHoward Chou, Ling Chen, Yunfeng Lei, Xiucai Liu
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Abstract
The invention provides microorganisms genetically modified to overexpress biofilm dispersal related polypeptides to enhance the production of lysine and lysine derivatives by the microorganism, method of generating such microorganism, and methods of producing lysine and lysine derivatives using the genetically modified microorganisms.
Claims12
A genetically modified host cell comprising an exogenous nucleic acid encoding a CsrB sRNA or a CsrC sRNA, wherein the host cell overexpresses the CsrB sRNA or CsrC sRNA relative to a counterpart host cell that has not been modified to express the exogenous nucleic acid;
- and overexpresses one or more proteins selected from the group consisting of a lysine decarboxylase, an aspartate kinase, a dihydrodipicolinate synthase, a diaminopimelate decarboxylase, an aspartate semialdehyde dehydrogenase, a dihydropicolinate reductase, and an aspartate transaminase to increase production of lysine or a lysine derivative compared to a wildtype host cell, wherein the host cell is Escherichia coli, and wherein the CsrB sRNA comprises a nucleotide sequence having at least 85% identity to SEQ ID NO:
- 16 and the CsrC sRNA comprises a nucleotide sequence having at least 85% identity to SEQ ID NO:
- 17.
A method of engineering a host cell to increase production of lysine or a lysine derivative, the method comprising introducing an exogenous nucleic acid encoding a CsrB or CsrC sRNA into the host cell, wherein the host cell overexpresses one or more proteins selected from the group consisting of a lysine decarboxylase, an aspartate kinase, a dihydrodipicolinate synthase, a diaminopimelate decarboxylase, an aspartate semialdehyde dehydrogenase, a dihydropicolinate reductase and an aspartate transaminase to increase production of lysine or a lysine derivative compared to a wildtype host cell;
- culturing the host cell under conditions in which the CsrB or CsrC sRNA is expressed, and
- selecting a host cell that exhibits increased production of lysine or a lysine derivative relative to a counterpart control host cell that has not been modified to express the exogenous nucleic acid, wherein the host cell is Escherichia coli, and wherein the CsrB sRNA comprises a nucleotide sequence having at least 85% identity to SEQ ID NO:
- 16 and the CsrC sRNA comprises a nucleotide sequence having at least 85% identity to SEQ ID NO:
- 17.
Dependencies are inferred from the claim text.