Method of meristem excision and transformation
AssigneeMonsanto Technology LLC
InventorsErik Dersch, Richard J. Heinzen, Brian J. Martinell, Anatoly Rivlin, Yuechun Wan, Xudong Ye
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Abstract
The present invention relates to excision of explant material comprising meristematic tissue from cotton seeds. Methods for tissue preparation, storage, transformation, and selection or identification of transformed plants are disclosed, as are transformable meristem tissues and plants produced by such methods, and apparati for tissue preparation.
Claims7
An apparatus for high-throughput generation of transformable plant tissue comprising a pair of spaced apart rollers comprising secondary grooves for applying a force to seeds passing through the rollers, further wherein the rollers of the pair are spaced from about 2.2 mm to about 3.5 mm apart, as measured by peak to valley of opposing rollers.
Dependencies are inferred from the claim text.