RNA polymerase variants for co-transcriptional capping
AssigneeModernaTX, Inc.
InventorsAthanasios Dousis, Kanchana Ravichandran, Amy E. Rabideau, Margaret Franklin, Kevin Smith, Michelle Lynn Hall
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Abstract
The present disclosure provides RNA polymerase variants for high efficiency transcription.
Claims31
A ribonucleic acid (RNA) polymerase variant comprising an RNA polymerase that comprises an amino acid substitution at position D351 relative to an RNA polymerase comprising the amino acid sequence of SEQ ID NO:
- 1, wherein the RNA polymerase variant has at least 95% identity to the amino acid sequence of SEQ ID NO:
- 1, and wherein the amino acid substitution at position D351 is selected from D351C, D351I, D351K, D351L, D351M, D351P, D351Q, D351R, D351S, D351T, D351V, and D351W.
A ribonucleic acid (RNA) polymerase variant comprising an RNA polymerase that comprises an amino acid substitution at positions E350 and D351, relative to a wild-type RNA polymerase comprising the amino acid sequence of SEQ ID NO:
- 1, wherein the RNA polymerase variant has at least 95% identity to the amino acid sequence of SEQ ID NO:
- 1.
A ribonucleic acid (RNA) polymerase variant comprising an RNA polymerase that comprises a C-terminal G and an amino acid substitution at positions G47, E350, and D351, relative to a wild-type RNA polymerase comprising the amino acid sequence of SEQ ID NO:
- 1.
A ribonucleic acid (RNA) polymerase variant comprising an RNA polymerase that comprises the amino acid sequence of SEQ ID NO:
- 126.
Dependencies are inferred from the claim text.