PSMA-targeted NIR dyes and their uses
AssigneeOn Target Laboratories, LLC
InventorSumith A Kularatne
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Abstract
The present disclosure relates to prostate specific membrane antigen (PSMA) targeted compounds conjugated to near-infra red (NIR) dyes and methods for their therapeutic and diagnostic use. More specifically, this disclosure provides compounds and methods for diagnosing and treating diseases associated with cells and/or vasculature expressing prostate specific membrane antigen (PSMA), such as prostate cancer and related diseases. The disclosure further describes methods and compositions for making and using the compounds, methods incorporating the compounds, and kits incorporating the compounds.
Claims20
A method of optical or diagnostic imaging of lung tissue that express prostate specific membrane antigen (PSMA) in a subject, wherein the lung tissue is selected from the group consisting of diseased lung tissue, abnormal lung tissue, lung tumor lesions, lymph nodes with metastatic lung tumor cells, primary lung cancer cells, and secondary lung cancer cells, the method comprising:
- contacting the lung tissue with a composition comprising a compound, or a pharmaceutically acceptable salt of the compound, wherein the compound has the formula:
- B—X—Y—Z wherein
- B comprises a compound capable of binding to PSMA;
- X is selected from the group consisting of polyethylene glycol (PEG), N-amino-dPEG2-acid, and polyethylene amine (PEA);
- Y comprises at least one amino acid, or a derivative thereof;
- and
- Z comprises a near-infrared (NIR) dye;
- illuminating the lung tissue with an excitation light of a wavelength absorbed by the compound;
- and
- detecting the optical signal emitted by the compound in the lung tissue.
A method of diagnosing lung cancer in a subject, the method comprising:
- a) administering to the subject in need of diagnosis a composition comprising a compound under conditions and for a time sufficient for the compound to bind and accumulate in lung tissue that express prostate specific membrane antigen (PSMA), wherein the lung tissue is selected from the group consisting of diseased lung tissue, abnormal lung tissue, lung tumor lesions, lymph nodes with metastatic lung tumor cells, primary lung cancer cells, and secondary lung cancer cells, the compound having the formula:
- B—X—Y—Z wherein
- B comprises a compound capable of binding to PSMA;
- X is selected from the group consisting of polyethylene glycol (PEG), N-amino-dPEG2-acid, and polyethylene amine (PEA);
- Y comprises at least one amino acid, or a derivative thereof;
- and
- Z comprises a near-infrared (NIR) dye;
- b) illuminating the compound to visualize the compound bound to the lung tissue using infrared light;
- c) measuring fluorescent signal from the illuminated compound bound to the lung tissue;
- d) comparing the fluorescent signal measured in step c) with at least one control data set, wherein the at least one control data set comprises a fluorescent signal from the compound contacted with a biological sample that does not comprise diseased lung tissue, abnormal lung tissue, lung tumor lesions, lymph nodes with metastatic lung tumor cells, primary lung cancer cells, and secondary lung cancer cells;
- and
- e) diagnosing the subject with lung cancer based on step d).
Dependencies are inferred from the claim text.