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US 11,484,040 B2Granted
Ampicillin resistant texturizing lactic acid bacteria strains
AssigneeChr. Hansen A/S
InventorsEric Johansen, Kim Ib Soerensen, Annette Kibenich
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Abstract
The present invention relates to mutants of lactic acid bacteria which are resistant to the antibiotic ampicillin and which were found to give an increased texture when grown in milk while maintaining the other growth properties of the parent strain. The present invention, furthermore, relates to compositions comprising such mutants, and to dairy products fermented with the lactic acid bacteria resistant to ampicillin.
Claims8
A method for obtaining a mutant texturizing lactic acid bacteria strain, comprising:
- (a) selecting and isolating, from a pool of lactic acid bacteria strains obtained from a mother strain without genetic engineering, a pool of mutant lactic acid bacteria strains which are resistant to the antibiotic ampicillin as determined by the amount of ampicillin that reduces the OD600 measured growth of the lactic acid bacteria strain, after 20 hours growth at 37° C. in a medium suitable for growth of the lactic acid bacteria strain, by 20% as compared to the OD600 measured growth in the medium without ampicillin being higher than 400 ng/ml;
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- (b) selecting and isolating from the pool of mutant lactic acid bacteria strains which are resistant to ampicillin a mutant lactic acid bacteria strain that exhibits a texturing property such that the efflux time of 28 ml of full fat cow milk containing 2% (w/v) skimmed milk powder, inoculated with at least 104 CFU/ml of the lactic acid bacteria strain and acidified at 37° C. for 20 hours, from a polystyrene 25-ml pipette is at least 50 seconds.
Dependencies are inferred from the claim text.