Image processing apparatus, evaluation system, image processing program, and image processing method

An evaluation system comprising:

• an imaging apparatus that captures a hologram in which a plurality of granules are contained within an imaging visual field;
• an image processing apparatus that performs image processing on the hologram;
• and
• an evaluation apparatus that outputs an evaluation result for each of the plurality of granules based on an image obtained by the image processing apparatus,
• wherein the image processing apparatus includes
• an acquisition unit that acquires the hologram,
• a generation unit that generates, from the hologram, phase difference images at positions different from each other in an optical axis direction in a case in which the hologram is captured,
• a specifying unit that specifies a plurality of image ranges in a direction of a plane intersecting the optical axis direction, which correspond to the plurality of granules, in an averaged image obtained by averaging at least some of the phase difference images, and
• an extraction unit that extracts the phase difference image at a center position of a corresponding granule in the optical axis direction for each of the plurality of image ranges, and
• the evaluation apparatus derives the evaluation result by using the phase difference image extracted by the extraction unit.

A non-transitory computer readable recording medium storing an image processing program causing a computer to execute a process comprising:

• acquiring a hologram obtained by imaging a plurality of granules contained within an imaging visual field;
• generating, from the hologram, phase difference images at positions different from each other in an optical axis direction in a case in which the hologram is captured;
• specifying a plurality of image ranges in a direction of a plane intersecting the optical axis direction, which correspond to the plurality of granules, in an averaged image obtained by averaging at least some of the phase difference images;
• and
• extracting the phase difference image at a center position of a corresponding granule in the optical axis direction for each of the plurality of image ranges.

An image processing method comprising:

• acquiring a hologram obtained by imaging a plurality of granules contained within an imaging visual field;
• generating, from the hologram, phase difference images at positions different from each other in an optical axis direction in a case in which the hologram is captured;
• specifying a plurality of image ranges in a direction of a plane intersecting the optical axis direction, which correspond to the plurality of granules, in an averaged image obtained by averaging at least some of the phase difference images;
• and
• extracting the phase difference image at a center position of a corresponding granule in the optical axis direction for each of the plurality of image ranges.