β-1, 6-glucanase mutant, and method for measuring β-1, 6-glucan
AssigneeToei Shinyaku Co., Ltd.
InventorsDaisuke Yamanaka, Naohito Ohno, Masuro Motoi, Akitomo Motoi
Life of this patent
Estimating the term…
Abstract
A β-1,6-glucanase mutant which is a mutant of β-1,6-glucanase (EC 3.2.1.75), wherein a Glu residue located at a position corresponding to Glu (E)-321 in SEQ ID NO: 1 is substituted by an amino acid residue X or a Glu (E) residue located at a position corresponding to each of Glu (E)-225 and Glu (E)-321 in SEQ ID NO: 1 is substituted by an amino acid residue X, wherein the amino acid residue (X) is selected from the group consisting of Gln (Q), Gly (G), Ala (A), Leu (L), Tyr (Y), Met (M), Ser (S), Asn (N), and His (H); and a method for measuring β-1,6-glucan, including measuring β-1,6-glucan bonded to the mutant.
Claims10
A β-1,6-glucanase mutant E321X which is a mutant of β-1,6-glucanase (EC 3.2.1.75), wherein a Glu (E) residue located at a position corresponding to Glu (E)-321 in SEQ ID NO:
- 1 is substituted by an amino acid residue X selected from the group consisting of Gln (Q), Gly (G), Ala (A), Leu (L), Tyr (Y), Met (M), Ser (S), Asn (N), and His (H).
A β-1,6-glucanase mutant E225X/E321X which is a mutant of β-1,6-glucanase (EC 3.2.1.75), wherein a Glu (E) residue located at a position corresponding to each of Glu (E)-225 and Glu (E)-321 in SEQ ID NO:
- 1 is substituted by an amino acid residue X selected from the group consisting of Gln (Q), Gly (G), Ala (A), Leu (L), Tyr (Y), Met (M), Ser (S), Asn (N), and His (H).
A β-1,6-glucan measuring kit containing a reagent (a) and a reagent (b), wherein reagent (a) is a β-1,6-glucanase mutant E321X which is a mutant of β-1,6-glucanase (EC 3.2.1.75), wherein a Glu (E) residue located at a position corresponding to Glu (E)-321 in SEQ ID NO:
- 1 is substituted by an amino acid residue X selected from the group consisting of Gln (Q), Gly (G), Ala (A), Leu (L), Tyr (Y), Met (M), Ser (S), Asn (N), and His (H), or a β-1,6-glucanase mutant E225X/E321X which is a mutant of β-1,6-glucanase (EC 3.2.1.75), wherein a Glu (E) residue located at a position corresponding to each of Glu (E)-225 and Glu (E)-321 in SEQ ID NO:
- 1 is substituted by an amino acid residue X selected from the group consisting of Gln (Q), Gly (G), Ala (A), Leu (L), Tyr (Y), Met (M), Ser (S), Asn (N), and His (H), and
- reagent (b) is a labeled mutant E321X obtained by adding a labeling substance to the β-1,6-glucanase mutant E321X, or
- a labeled mutant E225X/E321X obtained by adding a labeling substance to the β-1,6-glucanase mutant E225X/E321X.
Dependencies are inferred from the claim text.