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US 11,479,760 B2Granted
Biosynthetic platform for the production of cannabinoids and other prenylated compounds
AssigneeThe Regents of the University of California
InventorsJames U. Bowie, Meaghan Valliere, Tyler P. Korman, Nicholas Woodall
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Abstract
Provided is an enzyme useful for prenylation and recombinant pathways for the production of cannabinoids, cannabinoid precursors and other prenylated chemicals in a cell free system as well and recombinant microorganisms that catalyze the reactions.
Claims12
A recombinant polypeptide comprising a sequence selected from the group consisting of:
- (a) SEQ ID NO:30 and having at least a Y288X mutation, wherein X is A, N, S or V;
- (b) SEQ ID NO:30 having at least a Y288X mutation, wherein X is A, N, S or V, and at least one other mutation selected from V49Z1, F213Z2, A232S, I234T, V271Z3 and/or G286S, wherein Z1 is S, N, T or G, Z2 is H, N or G and Z3 is N or H;
- (c) a sequence of (a) or (b) further comprising from 1-20 conservative amino acid substitutions and having prenyltransferase (NphB) activity;
- and
- (d) a sequence that is at least 95%, 98% or 99% identical to SEQ ID NO:30 and which has at least the mutations of (a) or (b);
- wherein the polypeptide of (a)-(d) can perform prenylation reactions.
3 more elements
Dependencies are inferred from the claim text.