Method of improving methyltransferase activity
AssigneeDanmarks Tekniske Universitet
InventorsHao Luo, Anne Sofie Laerke Hansen
Life of this patent
Estimating the term…
Abstract
Methods for evolving cells or strains towards improved methyltransferase activity, particularly SAM-dependent methyltransferase activity, as well as to cells and strains useful in such methods and methods of using the evolved cells in the production of methylated products.
Claims13
A genetically modified E. coli cell comprising a s-adenosyl methionine- (SAM) -dependent methyltransferase, a heterologous cystathionine-beta-synthase selected from Saccharomyces cerevisiae, Streptomyces coelicolor, Streptomyces griseus, Clostridium acetobutylicum, Arabidopsis thaliana and Homo sapiens cystathionine-beta-synthase, and a heterologous cystathionine-gamma-lyase selected from Saccharomyces cerevisiae, Clostridium glutamicum, Streptomyces coelicolor, Streptomyces griseus, Bacillus subtilis and Homo sapiens cystathionine gamma-lyase, and
- (a) a downregulation or deletion of cysE;
- (b) a downregulation or deletion of cysK and cysM;
- (c) a downregulation or deletion of cysE and ilvA;
- (d) a downregulation or deletion of cysE and tdcB;
- (e) a downregulation or deletion of cysE, ilvA and tdcB;
- (f) a downregulation or deletion of cysE, ilvA, tdcB, and metA;
- (g) a downregulation or deletion of cysE, ilvA, tdcB, and metB;
- (h) a downregulation or deletion of metA, ilvA and tdcB;
- (i) a downregulation or deletion of metB, ilvA and tdcB;
- (j) a downregulation or deletion of metC, malY, ilvA and tdcB;
- (k) a downregulation or deletion of metC, malY, ilvA, tdcB and metA;
- (l) a downregulation or deletion of metC, malY, ilvA, tdcB and metB;
- or
- (m) any one of (a) to (l), further comprising a downregulation or deletion of cfa,
- wherein, in (a) to (m), the downregulation of a gene reduces the level of enzyme encoded by the gene by at least 95%.
Dependencies are inferred from the claim text.