Methods and compositions for modulating complex mixtures by selective deletion chromatography

A method of preparing a therapeutic or nutraceutical composition for human or animal administration, the therapeutic or nutraceutical composition comprising an edited extract composition prepared from an original composition, the method comprising:

• applying an extract sample from the original composition to a liquid chromatography system comprising a chromatography column;
• applying a mobile phase to the chromatography column after the extract sample is applied to the chromatography column;
• dividing a total eluant volume into a first eluant stream and a second eluant stream, wherein the first eluant stream comprises a minority fraction by volume of the total eluant volume, and wherein the second eluant stream comprises a majority fraction by volume of the total eluant volume;
• directing the first eluant stream to a detector configured to identify components therein, wherein a detection signal associated with identified components is related to an elution time in the first eluant stream;
• collecting the second eluant stream, wherein the second eluant stream is collected in a first collected fraction for a first given length of time, wherein the second eluant stream is diverted and collected in a second collected fraction for a second given length of time subsequent to the first given length of time, wherein the second given length of time corresponds to a component and elution time identified in the first eluant stream by the detector, and wherein after the second collected fraction is obtained, the second eluant stream is returned to collection in the first collected fraction for a third given length of time, thereby providing the edited extract composition with one or more components omitted from the original composition;
• and
• formulating the therapeutic or nutraceutical composition for human or animal administration comprising the edited extract composition.