High-throughput block optical DNA sequence identification

A method for identification of nucleotide content in a portion of a polynucleotide, comprising:

• treating the polynucleotide to generate a polynucleotide fragment;
• applying the polynucleotide fragment to a surface;
• establishing a Ramen spectroscope and a Fourier transform infrared (FITR) spectroscope;
• directing a light source with a wavelength toward at least a portion of the polynucleotide fragment, wherein the portion comprises between 2 and 20 nucleotides;
• allowing the light to interact with the portion of the polynucleotide fragment;
• detecting light reflected by the portion of the polynucleotide fragment using said Ramen spectroscope and a Fourier transform infrared (FITR) spectroscope;
• determining an intensity of the Raman shift of the reflected light from about 200 to about 1500 cm-1;
• determining and amount of absorbance from about 600 to about 1800 cm-1;
• measuring the intensity of Raman shift at one or more wavenumbers between 200 and 1500 cm-1 and calculating an area under a curve for each measured wavenumber;
• determining the relative content of adenine, thymine, cytosine, and guanine in the portion based on the relative intensity of the one or more wavenumbers;
• thereby
• identifying the nucleotide content in the portion of the polynucleotide fragment.