Aminopeptidases for protein hydrolyzates
AssigneeDupont Nutrition Biosciences Aps
InventorsKarsten Matthias Kragh, Ernst Meinjohanns, Thomas Eisele, Peter Edvard Degn, Steffen Yde Bak
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Abstract
The present disclosure provides polypeptides having aminopeptidase activity and isolated nucleic acid sequences encoding the polypeptides. In some embodiments, the disclosure also provides to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing the polypeptides. In some embodiments, the present disclosure further provides to methods of obtaining protein hydrolysates useful as flavor improving agents.
Claims6
A method for the production of a protein hydrolysate comprising subjecting a proteinaceous substrate, wherein the substrate is selected from the group consisting of casein, whey protein isolate, soy protein isolate and gluten, to a purified polypeptide having aminopeptidase activity, selected from the group consisting of:
- (a) a polypeptide having an amino acid sequence comprising the amino acid sequence of SEQ ID NO:
- 1, or SEQ ID NO:
- 2, or SEQ ID NO:
- 3, or SEQ ID NO:
- 4, or SEQ ID NO:
- 5, or SEQ ID NO:
- 6, or SEQ ID NO:
- 7, or SEQ ID NO:
- 8;
- and
- (b) a fragment of (a), wherein the fragment has aminopeptidase activity.
A method for obtaining from a proteinaceous substrate a protein hydrolysate enriched in free glutamic acid and/or peptide bound glutamic acid residues, comprising subjecting the substrate to a deamidation process and a purified polypeptide having aminopeptidase activity, selected from the group consisting of:
- (a) a polypeptide having an amino acid sequence comprising the amino acid sequence of SEQ ID NO:
- 1, or SEQ ID NO:
- 2, or SEQ ID NO:
- 3, or SEQ ID NO:
- 4, or SEQ ID NO:
- 5, or SEQ ID NO:
- 6, or SEQ ID NO:
- 7, or SEQ ID NO:
- 8;
- and
- (b) a fragment of (a), wherein the fragment has aminopeptidase activity.
Dependencies are inferred from the claim text.