Method for obtaining microglia from pluripotent stem cells
AssigneeTakara Bio Inc.
InventorHiroki Saito
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Abstract
The purpose of the present invention is to efficiently produce microglia from pluripotent stem cells. Provided is a method for producing microglia from pluripotent stem cells, comprising the following steps: (a) a step of co-culturing a pluripotent stem cell together with a feeder cell for 7 days or longer, and obtaining a blood progenitor cell; (b) a step of co-culturing the blood progenitor cell obtained in step (a) together with a feeder cell in the presence of IL-3 and/or GM-CSF, and obtaining an embryonic monocyte; and (c) a step of, in the presence of M-CSF, co-culturing the embryonic monocyte obtained in step (b) together with an astrocyte, or culturing the embryonic monocyte using an astrocyte supernatant.
Claims8
A method for producing microglia from a pluripotent stem cell, the method comprising the following steps:
- (a) a step of co-culturing a pluripotent stem cell with a feeder cell for 7 days or more to obtain a blood progenitor cell,
- (b) a step of co-culturing the blood progenitor cell obtained by step (a) with a feeder cell in the presence of IL-3 and/or GM-CSF to obtain a primitive monocyte, and
- (c) a step of co-culturing the primitive monocyte obtained by step (b) with an astrocyte in the presence of M-CSF, or culturing the primitive monocyte obtained by step (b) using an astrocyte supernatant in the presence of M-CSF.
Dependencies are inferred from the claim text.