Virus-free cell lines and methods for obtaining same
AssigneeGlycobac, LLC
InventorsAjay Maghodia, Christoph Geisler, Donald Jarvis
Life of this patent
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Abstract
The current teachings are directed to virus free cells lines derived from virus-contaminated starting material, such as an organism or a cell line. Methods for obtaining virus free cell lines obtained from virus-contaminated starting material are also provided. Exemplary virus free cell lines include: cell lines derived from a Spodoptera frugiperda cell line contaminated with Sf-rhabdovirus, wherein the cell lines lack Sf-rhabdovirus; and cell lines derived from a Trichoplusia ni cell line contaminated with an alphanodavirus, wherein the cell line lacks an alphanodavirus.
Claims4
A method for obtaining a Sf-rhabdovirus free Spodoptera frugiperda cell line, comprising:
- isolating a cell from a Sf-rhabdovirus-contaminated Spodoptera frugiperda organism or from a Sf-rhabdovirus-contaminated Spodoptera frugiperda cell line, wherein isolating comprises limiting dilution;
- combining the isolated cell with a cell culture media comprising an antiviral compound to form a first culture composition, wherein the antiviral compound is 6-azauridine;
- incubating the first culture composition under conditions suitable for the cell to grow and divide, thereby generating a multiplicity of cells;
- removing a portion of the multiplicity of cells or the cell culture media and testing for the presence or absence of a virus;
- combining at least some of the multiplicity of cells with cell culture media without an antiviral compound to form a second culture composition;
- and
- incubating the second culture composition under conditions suitable for the cells to grow and divide, thereby obtaining the Sf-rhabdovirus free cell line.
Dependencies are inferred from the claim text.